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Epitalons ability to reactivate telomerase has been demonstrated using the Telomeric Repeat Amplification Protocol (TRAP) assay in two key cell models: HeLa cells (telomerase-positive cancer cell line) and Human fetal lung fibroblasts (602/17)
0344 Bibliografia -Hiatt WR, Regensteiner JG, Creager MA, Hirsch AT, Cooke JP, Olin JW, Gorbunov GN, Isner J, Lukjanov YV, Tsitsiashvilli MS, Zabelskaja TF
Observed outcomes include specific cellular and molecular endpoints such as: Enhanced fibroblast migration capacity Increased collagen and elastin deposition Modulation of pro-inflammatory and antioxidant mediators Altered transcriptional profiles related to tissue homeostasis, repair signaling, and stress response All reported observations derive exclusively from preclinical laboratory and animal studies and remain model-specific
Population Doubling Level Calculation To calculate the PDL, a formula was used as follows: n = 3.32 (log UCY log I ) + X , where n is the final PDL at the end of a given subculture, UCY is the cell yield at that point, I is the cell number used as inoculum to begin that subculture, and X is the PDL of the inoculum used to initiate the subculture being quantitated (Pham et al., 2020)
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