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The enzymatic activities of these two enzymes were differentially regulated

The following six asynchronous mutations were found: V12M , Q126X , Q141K , G268R , S441N , and F506SfsX4 (Matsuo et al., 2009)

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pAAV-hSyn-EGFP, plasmid from Addgene, 50465-AAV8, RRID: Addgene_50465-AAV8, virus packaged at UNC Vector Core (100 l at titre 7 10 12 vg ml 1 ), retroAAV2-EF1a-oNLS-oScarlet 52 (100 l at titre 3 10 13 vg ml 1 )), was delivered using a glass pipette at a flow rate of 50 nl per min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4) per targeted site