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Subsequently, cumulus-oocyte complexes (COCs) with a diameter of about 100130 m and a central oocyte surrounded by at least three layers of cumulus cells were collected according to the department's protocols ( After collection, COCs were transferred to 500 l of IVM droplets (4050 COCs per droplet) covered by mineral oil and cultured for a subsequent 24 h in a CO 2 incubator (38.5C and 5% CO 2 )

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