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Some efforts have been made to solve this issue, such as the application of band-selective optimised flip-angle short-transient (SOFAST) HMQC techniques to 15 N-labelled cells, which provide high-resolution spectra of small metabolites directly in living cells in a few seconds (Motta et al., 2003)
The different developmental stages utilize various enzymes synthesized by the mycelia, which depend on growth conditions and substrate utilization
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