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Depress the plunger slowly, letting the water trickle down the inside wall of the vial rather than hitting the lyophilized powder directly

Standard volumes are: 2.5 mL standard (2 mg GHK-Cu per 0.1 mL draw) 5 mL for a 0.2 mL (20-unit) draw easier syringe-mark reading and reduced concentration-dependent sting Let the water run down the inside vial wall, then swirl gently without shaking