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Elevated superoxide generated from dysfunctional mitochondria promotes the formation of excessive H 2 O 2 , the substrate for MPO-mediated hypochlorous acid synthesis and the generation of the inflammatory biomarker, 3-CT
Then 20 l serum was mixed with 2 l 200 mM NEM (Thermo Scientific, 040526.06) and extracted with 80% methanol
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Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantitative methods are one of the most commonly used techniques, with the advantage of being able to efficiently separate and detect a variety of carnitine and its derivatives, including free carnitine, acylcarnitine, and their isomers and odd forms
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