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Trypsin/LysC digestion and streptavidin enrichment Samples were digested by adding 3 l of 100 mM CaCl 2 (final concentration: 1 mM) and 2 g of trypsin/LysC (20 g trypsin/LysC resuspended in 40 l trypsin/LysC buffer, then adding 4 l to each sample), followed by shaking at 37 C for 16 hours
But electrolytes are not a magic fix for every GLP-1 side effect
For instance, GLP-1 treatment attenuates the endotoxin-induced release of the potent proinflammatory cytokine IL-1 [71], which is known to inhibit neuronal transmission and promote apoptosis [72]
Some patients feel anxious, on edge, or unable to relax
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