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Fontagn Dicharry et al., 2015
I bought a good stock, (stored at the top o the fridge), in anticipation of Brexit, which have been out of date for a while, and have been using them every other day for a while now with no problems
The pellet was resuspended in 2 ml RPMI-MGIA, adjusting splenocyte concentration to 3x10 6 or 5x10 6 in 600l for co-culture with BCG (300 or 500 CFU) in 48-well plates, and incubated for 96 hours at 37C with CO 2
The pathological effects of oxidative stress on spermatozoa can be grouped into three main categories: lipid peroxidation, DNA fragmentation, and protein oxidation
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