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25 mM HEPES-KOH, pH 7.4, 225 mM mannitol, 1 mM EDTA) containing 30% Percoll and centrifuged at 95,000 g for 30 min at 4 C using a swing rotor
Store out of direct sunlight in dry place 65-80F (20-25C)
the aqueous solutions can also be decolourised and further purified with activated carbon, diatomaceous earths or other inert adsorbent material
It has been a subject of in vitro research related to cellular signaling, matrix metalloproteinase activity, and gene expression regulation
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