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The strongest predictors are baseline IGF-1 level, age, sleep quality (7-9 hours nightly), training consistency (4-6 days/week), diet quality, and protocol adherence

Finally, microbial functional profiling was carried out using HUMAnN 3.5 [34], through which the gene families were grouped into one feature table, normalized into copies per million (CPM), and further grouped in Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologs [35,36,37], and pathways

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