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[15] At the end of this feeding period, half the HFD-fed mice were randomly assigned to receive berberine
While our immunofluorescence data and neural fiber labeling support a functional connection between the NTS and ARC, definitive monosynaptic tracing using transsynaptic viral tools in genetically defined cre lines remains technically demanding and was not feasible in this phase of the study
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In addition, cumulative evidence suggests that KOA as a metabolic disorder also requires energy metabolic pathways to meet the demands of tissue repair
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