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Gene expression analysis Total RNA from cell lines was purified using QIAwave RNA Mini Kit (74536, QIAGEN) and total RNA from murine tissues was isolated by trituration with a motorized micro pestle, followed by column purification with QIAwave RNA Mini Kit (74536, QIAGEN)

Author contributions YL: Data curation, Methodology, Investigation, Writing review & editing, Formal analysis, Writing original draft, Project administration

In muscles, the spinal cord, and the brain, the liver enzyme glutamine synthetase converts glutamate and ammonia (a toxic byproduct) into glutamine, protecting against ammonia toxicity