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Cells were then washed in incubation buffer (1% BSA in 1X PBS) and stained for myogenic lineage using 1 g of CD56 primary antibody conjugated to PE/Cyanine5 (BioLegend, San Diego, CA, United States, Cat

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Day 1: 500mg or 1000mg NAD IV (2.5-5 hrs) Day 4: 100mg NAD SQ Day 6: 100mg NAD SQ Daily: 500 mg NR/NMN in the morning Week 9 onwards (Maintenance) We split the two week period in week A and week B and cycle continuously between the two