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Cryo-EM Three microliters of the purified GLP-2GLP-2RG s complex at about 20 mg/mL was applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), blotted, and subsequently sample-coated grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific, Waltham, USA)
In the CT-like conformation, the bypass motif becomes unstructured, and residues (S21-P28) are not well resolved, suggesting this flexible segment is exposed to the solvent environment
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