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For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)
Int J Obes (Lond) (2015) 39(1):187
1.2.2 Housing: General procedures for animal care and housing were in accordance with the standard, Commission on Life Sciences, National Research Council, Standard operating procedures (SOPs) of Pharmaron, Inc
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