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Western Blotting Analysis Proteins from the whole kidney lysates were separated on a 10% SDS-polyacrylamide gel, transferred onto PVDF membrane, blocked in 5% BSA for 60min, and then primary antibodies against KIM-1, IL-1, TNF-, Ferritin, FPN, xCT, and p53 at the dilution of 1:1,000, as well as 4-HNE, GPX4, and -actin at the dilution of 1:4,000 were probed at 4C overnight
We do this to make sure the plasma we collect is top-notch and safe for those who need it
On the other hand, the formation of Lewy bodies takes place through complex processes, and the role of Lewy bodies in the occurrence of alpha-synucleinopathies needs complex explanations [38]
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