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Analysis of acyl distribution in TAGs Four freezethaw cycles were performed on cell pellets obtained from 800 mL of broth, followed by ultrasonication using an ultrasonic homogenizer (Ningbo Scientz, China)
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It incorporates key modifications, including 14E/17R mutations to stabilize an -helical segment via a salt bridge, multiple proline substitutions (25P, 28P, 29P) to reduce -sheet formation, and N-terminal C20 fatty diacid lipidation for reversible albumin binding (Kruse et al
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