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As a reminder, in humans, eight different isoforms of GPx (GPx18), which use GSH to catalyze the reduction of hydrogen peroxide and lipid peroxides, have been identified [19, 20]: GPx1, GPx2, GPx3, GPx4, and GPx6, which contain a selenocysteine residue (SeCys) and the GPx5, GPx7, and GPx8 that do not contain SeCys but contain a Cys residue
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Viability of spheroids were determined using CellTiter-Glo 3D cell viability assay kit (Promega)
Ryter SW, Otterbein L, Morse D, Choi AM: Heme oxygenase/carbon monoxide signaling pathways: regulation and functional significance
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