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After incubation, samples were rinsed with 0.1% (w/v) BSA (A9647, Sigma) in PBS 1 and centrifuged at 1200 rpm for 3 min

14 One study, conducted in Europe, found the following averages for IGF-1 levels in healthy patients of different age ranges: 15 The study reported an average serum IGF-1 level of 200-210 ng/ml, suggesting that this is the typical level for adults on a Western diet