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When NSBSO is reduced by GSH, the disulfide bond of NSBSO will break and NSBSO will then be converted to Nap- D F D FY-thiol and this process can be detected by liquid chromatography-mass spectrometry (LCMS) analysis (Additional file 1: Fig
Similarly, H7, A8 and F12 of GLP-1 interact with E 364 and F 367 of TM6 of GLP-1R, whereas, Igl2 of SR18 forms hydrophobic interactions with F 367
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