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As reported for nitric oxide synthase whose K M value for arginine is much greater in cells than that in assay tubes (Wu and Meininger 2000), it is possible that the K M of carnosine synthetase for histidine in vivo is much greater than that (i.e., 16.8 M) reported for the purified enzyme under in vitro assay conditions, due to complex proteinprotein and enzymesubstrate interactions in vivo
doi: 10.1101/2024.02.08.579493 18
This inconsistency made it difficult to maintain adherence and achieve the best possible health outcomes
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