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Sections were fixed with 4% paraformaldehyde, rinsed with PBS, blocked with goat serum (Vector laboratories, CA, USA) for 30 min at room temperature, and incubated with mouse-anti-GSK-3/ monoclonal antibody (SC-7291, 1:50, Santa Cruz, TX, USA) at 4 C overnight
Historically, vitamin C was recognized as the perfect treatment and prevention of scurvy , a disease that decimated ascorbic acid-deficient people between the 1500 and 1800s
Dupr, A
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