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Is 9mg a better maintenance dose than 12mg
Briefly, i4F MEFs were seeded at a density of 3 10 5 cells per well in six-well tissue culture plates coated with gelatin and treated with doxycycline (PanReac, A2951) 1 mg ml 1 continuously to induce expression of the OSKM transcription factors in the presence of complete KSR media (15% (vol/vol) Knockout Serum Replacement (KSR, Invitrogen, 10828028) in DMEM with GlutaMax (Life Technologies, 31966047) basal media, with 1,000 U ml 1 LIF (Merck, 31966047), non-essential amino acids (Life Technologies, 11140035) and 100 M beta-mercaptoethanol (Life Technologies, 31350010) plus antibiotics (penicillinstreptomycin, Gibco, 11528876)), which was replaced every 4872 h
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