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Muscle tissues were collected from rats and mice after euthanasia with an overdose of isoflurane in an induction chamber

Calming Choose Semax When Research Requires: Cognitive enhancement and improved focus BDNF upregulation for neuroplasticity research Neuroprotection in stroke or ischemia models Dopaminergic modulation studies Learning and memory enhancement Choose Selank When Research Requires: Anxiolytic effects without sedation GABAergic modulation without benzodiazepine-type effects Stress response attenuation Immunomodulatory peptide research Non-dependence-forming anxiolytics Consider Combination When: Balanced cognitive enhancement with mood stabilization is desired Research subjects may experience overstimulation from Semax alone Both neuroplasticity and stress resilience are targets Regulatory Reality: Both peptides are approved medications in Russia but have not undergone Western regulatory approval

ApopTag Peroxidase kits (Merck Millipore, Bayswater, VIC, Australia) were used for TUNEL staining