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Validation experiments established that cells expressing T7 RNAPlinkerT7 lysozyme fusions containing various substrate sequences exhibited growth rates in the presence of chloramphenicol that correlate with the proteases known activity on that substrate (Supplementary Fig
consultation required to identify protocol fit
At confluence, chondrocytes were treated with GS (Rottapharm, Monza, Italy) at the indicated concentrations for 20 h [for messenger RNA (mRNA) determination] or 72 h (for protein determination) in DMEM containing 0.5% FBS in the presence or absence of IL-1 100 pg/ml
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