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The arena used was the open field, with mice habituated to the empty area for 5 min the day before the NOR test
IGF-1 is responsible for many of the effects of GH, such as increasing muscle mass, reducing body fat, and promoting bone growth, among others
Caffeine is well known for increasing energy expenditure and fat oxidation
It is studied in research into fat oxidation, body weight reduction and metabolism, via inhibition of the enzyme NNMT and the NAD+ routes connected to it
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