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Thereafter, anhydrous sodium sulfate was added to remove moisture from the product which was then filtered and distilled under reduced pressure to remove ethyl acetate, followed by fractional distillation under reduced pressure (93-95 C/5 mmHg) to obtain 40.4 g (yield: 80 %) of ethyl 4-chloro-3-hydroxybutyrate

Analysis of single-cell RNA sequencing Single-cell RNA sequencing (scRNA-seq) data (HRA000425) were downloaded from Genome Sequence Archive (SRA)-Human databases in China National Center for Bioinformation 16 , converted into FASTQ format using fasterq-dump software (sratoolkit 3.0.2, NCBI, NIH, Bethesda, MD, USA), converted into a matrix data file using Cell Ranger software version 3.0.2 (10xGenomics, Pleasanton, CA, USA), converted into Seurat objects using Seurat 5.0.1 software (Satija lab, Lower Manhattan, New York, NY, USA), and plotted for t -distributed stochastic neighbor embedding ( t -SNE) visualization

the Natural Science Foundation of Shanghai (23ZR1475200 to LHZ)