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To determine creatinine clearance, mice were housed individually in metabolic cages for 24 h urine collection at the end of the treatment period
During the oxidation phase, accumulated ROS (such as hydrogen peroxide HO and superoxide anion O) in the cell can directly react with GSH in a non-enzymatic manner, or, under the catalysis of glutathione peroxidase (GPX), promote the oxidative coupling of two GSH molecules to form one molecule of GSSG, while releasing two protons (H) and two electrons (e)
Cells within three random fields for each condition were counted using a Zeiss microscope in a double-blind manner
Glutathione and other thiols in biological samples can interfere with the assay
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