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Typically, top biomarker candidates identified from screening studies will be validated through a combination of secondary focused screens and individual gene perturbation studies which include independent sgRNAs, rescue experiments, and functional assayssuch as proliferation, apoptosis, or immune cell co-cultureto confirm their role in tumor biology
Inhibition of caspase-3 activity and activation by protein glutathionylation
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Human evidence is therefore rated Low quality overall
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