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Proteins (50 g) from PMS or mitochondrial extract from ASA/NAC treated GSH-depleted HepG2 cells were separated on 12% SDS-PAGE and transferred on to nitrocellulose paper by Western blotting as described in the Materials and Methods
Epithelial loss of ClpP was confirmed by qPCR analysis of ClpP mRNA in isolated IECs ( Figure 5A )
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