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Reduced glutathione (GSH) can react with dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly
TUNEL assay On experimental day P38, pancreas samples were fixed in 2% w/v paraformaldehyde for 2 h and processed for TUNEL assay, which was performed using the One Step TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China) according to the manufacturers instructions
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