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For fixation, an equal volume of PBS (Thermo Fisher Scientific) + 8% paraformaldehyde (Electron Microscopy Sciences) was added to the medium for 15 min, after which the samples were washed three times with PBS

Methotrexate is metabolized and stored in hepatocytes in its polyglutamated form, leading to a longer presence of MTX within the cells, which has been suggested as a possible mechanism for MTX-induced liver damage (Bath et al

Specific Nutrient Supplements : Depending on individual needs, specific supplements like vitamin D, calcium, iron, and B vitamins can help

The No DAC form sits at approximately 3367 g/mol