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For maximum accuracy at these concentrations, consider using a 0.5 mL insulin syringe with half-unit markings
Crystal Broussard, MD) Dr
All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction
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