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Then, the cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford a protein free lysate

These interactions involve signal transduction, membrane trafficking, cytoskeleton, metabolic cross-talk, and control of programmed host cell death