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Why yours is low
It undermines consistency across the entire protocol
W = A or T), which is present in the promoter region of gtmA and all gli genes, except gliZ and gliA , and is very similar to the TCGGNNNCCGA sequence reported previously by others investigators (Fox et al., 2008)
The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates
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