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The resin was resuspended by 2 mL wash buffer C and the TEV protease was added to remove the N-terminal tag at a molar ratio of 1:10 and the mixture was shaken at 4 C for at least 16 h
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Sometimes realistic protein matters more than ideal protein. If you want a list of protein-rich recipes, check out my round-up of GLP-1 recipes
2019;32:e13049
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