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Additionally, 4-hydroxy-nonenal (HNE) and Isoprostanes (F2-isoprostane, 15-(S)-8-isoprostagladin F2) are by-products of PUFA, and are also considered as indices of lipid peroxidation ( Total Antioxidant Capacity TAC highlights the crucial role of antioxidant enzymes in counterbalancing ROS generation and therefore can be a powerful tool in determining the redox status of a sample ( Oxidative Reduction Potential Oxidative reduction potential (ORP) is a direct analysis of OS ( ORP has been shown to be negatively correlated with sperm concentration and total sperm count ( This analysis does not rely on any biomarker for OS and allows for the quantification of all oxidants and antioxidants in a given sample ( The Male infertility Oxidation System (MiOXSYS) can be used to accurately measure ORP ( The system consists of an electrochemical cell with a counter and a reference electrode, as well as an impedance electrometer ( It measures static ORP (sORP) which is indicative of the existing balance between total oxidants and reductants in a sample, and the antioxidant capacity reserve (cORP)