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gambiae infected midguts after 60 min of incubation in 6 M HCl was fixed in 2.5% (v/v) glutaraldehyde, 3mM MgCl 2 in 0.1 M sodium cacodylate buffer, pH 7.2 for 1 h at room temperature
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They retained a fragment of their original genome, a circular strand of DNA encoding 37 genes
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