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RNA extraction and real time quantitative PCR Total RNA was isolated from liver tissue using Trizol (Roche, Mannheim, Germany) in accordance with manufacturers instructions and was converted to cDNA using a previously described method 5

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Different from regular cancer cells, CSCs have a lower level of ROS, which might contribute to a slower growth rate, reduced oxidative metabolism, and elevated expression of the ROS scavenging system (Hyewon and Navdeep, 2018)