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Krka, Slovenia)), we induced abdominal compartment syndrome by intraperitoneal insufflation of ordinary air controlled by a manual and digital manometer with a data logger connected to a computer (DD890, Dostmann Electronic GmbH, Germany) and maintained high abdominal pressure at 25 mmHg for 120 min before sacrifice, with a pressure measurement interval of 1 s

9 Twin studies suggest a genetic component may also be a factor

Both routes should be paired with an independent measurement of copper by an elemental method, because copper quantitation answers a question no peptide-based method can: how much metal is present, as distinct from how much ligand