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Description

The specific parameters for Trimmomatic are: Truseq3-SE.fa:2:30:10 --LEADING:3 --TRAILING:3 --SLIDINGWINDOW:4:1 --MINLEN:36.The specific parameters for STAR are: --outSAMunmapped Within --outFilterType BySJout --outSAMattributes NH HI AS NM MD --outFilterMultimapNmax 20 --outFilterMismatchNmax 999 --outFilterMismatchNoverLmax 0.04 --alignIntronMin 20 --alignIntronMax 1000000 --alignMatesGapMax 1000000 --alignSJoverhangMin 8 --alignSJDBoverhangMin 1 --sjdbScore 1 --runThreadN 12 --genomeLoad NoSharedMemory --outSAMtype BAM Unsorted --quantMode TranscriptomeSAM --outSAMheaderHD \@HD VN:1.4 SO:unsorted

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