sglt2 glp 1 combination Diabetic Agents, From Metformin to Inhibitors and GLP1 Receptor Agonists: JACC Focus Seminar SGLT2 inhibitors and GLP-1 receptor

This work was supported by a National Priorities Research Program grant (NPRP 14 S-0311210033

Each of the criteria was scored semiquantitatively as 0: none

Eaton SB, Konner M

To isolate intestinal organoid, following euthanasia of 810-week-old male mice, crypts were isolated from the ileum fragments by EDTA incubation and plated in Matrigel using advanced DMEM/F12 containing 100 U/mL penicillin-streptomycin, 10 mM HEPES, 2 mM GlutaMAX (35050061), supplements N2 (17502048) and B27 (17504044) from ThermoFisher, 50 ng/mL murine EGF (315-09, Peprotech), 1 mmol/L N-acetylcysteine (A9165, Sigma), 100 ng/mL murine Noggin (250-38, Peprotech), and 1000 ng/mL human R-spondin-1 (120-38-20, Peprotech)