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PMID: 29939616

This review emphasizes the necessity for adopting a comprehensive approach that combines molecular and cellular knowledge with practical methods in the field of sperm cryopreservation to ensure production of goats as major food animals in the global scale

a soluble cytoplasmic protein)), you break open the cells (lyse them), spin that lysate super fast in a centrifuge to pellet out the insoluble stuff (membrane bits, etc.), and then purify the protein of interest from the liquid part (supernatant) containing the soluble things