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The missing variable might not be your protocol at all - it might be what's on your plate
Tierney, M
The N-terminally located centre (Cys59 hTrxR1 , Cys64 hTrxR1 ) is buried in the protein, whereas the second, C-terminally located centre (Cys497 hTrxR1 , Sec498 hTrxR1 ) is positioned on a flexible, highly accessible C-terminal arm of the other subunit (residues belonging to the other subunit are primed)
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