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Ignoring the solvent control, it was claimed that pretreatment with the pancaspase inhibitor Z-VAD-fluoromethylketone protected against APAP toxicity despite lack of caspase activation [41]
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Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the StepOne Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA) and THUNDERBIRD SYBR qPCR Mix (Toyobo)
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