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Because in killifish there are no validated antibodies that can be used as bona fide surface markers to distinguish among specific immune cell clusters, we took advantage of the variation in the biophysical properties in the cell populations extracted from the kidney marrow and used cytometry to identify separate cells based on cellular complexity (side scatter (SSC)) and cell area (forward scatter (FSC))
Even gradual dose escalation does not eliminate intolerance in every patient
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