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The chemokine CCL2 mediates migration that increased BBB permeability, as shown when HIV-1 infected lymphocytes were added to transwells with astrocytes and BECs (Eugenin et al., Cancer Cells Malignant glioma cells infiltrate peri-vascular spaces and displace astrocytic end-feet from BECs (Cuddapah et al., 2014)

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BALF was centrifuged to collect supernatants for cytokine and total protein content analysis, while cell pellets were used for differential cell counts via cytospin centrifuge and Hema-3 staining

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