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Mild appetite increase
Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates
Four carbon hydrogen bonds were observed with VAL22 (3.03 ), SER274 (2.76 ), PRO357 (3.16 ), and HIS226 (2.84 )
Importantly, these reactions do not usually mean an allergy to the medication
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