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The readings were expressed as fluorescent units per mg of protein and converted to percentage by taking normoxic value as 100%

Proper storage ensures reproducibility: Unopened kit : Store at 28C Opened kit : Follow protocol-specific storage instructions Shipping : Gel pack with blue ice maintains temperature integrity during transit Critical handling tips from the manufacturer: Allow all reagents to warm to room temperature for at least 30 minutes before opening Pre-rinse pipette tips with reagent and use fresh tips for each sample, standard, and reagent Mix thoroughly use a low-frequency oscillator or gentle hand shaking every 10 minutes Run samples and standards in duplicate or triplicate Keep unused wells desiccated at 4C in the provided sealed bag Is cross-reactivity a concern

2 and 3), demonstrating that the disruption of the auxin homeostasis at the transcript level occurs ahead of the root tip enlargement