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The antibiotic-resistant gene was removed from the genomic DNA of W303-1b/F using the Cre- loxP system, then W303-1b/F cells were further transformed with the linearized recombinant plasmid pGAPg-gshF to obtain engineered strain W303-1b/FF (Table 2)

173 At the molecular level, gene modulation techniques, including siRNA- and shRNA-mediated silencing of efflux transporter genes (e.g., ABCB1, ABCG2), as well as CRISPR/Cas9-based gene editing, have successfully resensitized tumor cells to chemotherapy in preclinical models

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Its enhanced bioavailability ensures that these benefits are delivered more effectively than traditional IGF-1, providing sustained support for overall well-being and performance optimization